Studies on tetraspores shedding in padina tetrastromatica. Read effect of ascophyllan from brown algae padina tetrastromatica on inflammation and oxidative stress in carrageenaninduced rats, inflammation on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Journal of environmental sciences 2010, 229 141417 assessment of oxidative stress indices in a marine macro brown alga padina tetrastromatica hauck from comparable polluted coastal regions of the arabian sea, west coast of india. Also, the phytochemical composition of the algae is known. The mean zone of inhibition produced by the extracts in disc diffusion assays against the tested bacterial strains ranged from 7. Sterile glass powder was prepared by crushing broken borosilicate glass to a fine powder using a mortar and pestle. Extraction buffer was prepared having the following composition 100 mm trishcl, 20 mm edta, 1. Padina tetrastromatica showed 50% of activity from the concentration at 1. The present study deals with the synthesis of silver nanoparticles agnps using the aqueous extract of brown seaweed padina tetrastromatica leaf extract. Three species of seaweeds padina tetrastromatica, caulerpa. Sulfated polysaccharides purified from two species of padina improve collagen and epidermis formation in the rat.
The main fatty acids in the lipids were also identified. Padina tetrastromatica hauck dictyotales, phaeophyta to study the compositional changes and the probable binding sites of cd using ftir technique. The potential for using cyanobacteria blue green algae and algae in the biological control of plant pathogenic bacteria and fungi martin m. The polysaccharides were purified from aqueous extracts of p. In this study, lcmsmsbased structural characterisation of phlorotannins from padina tetrastromatica, a marine brown macroalga collected from southwest coastal region of kerala, and its bioactivities are presented. Antioxidant and cytotoxic activities of three species of. Padina, a genus of brown algae disambiguation page providing links to topics that could be referred to by the same search term this disambiguation page lists articles associated with the title padina. Padina antillarum is widely distributed in the indian ocean silva et al. Total free amino acid content of freshly collected frozen tissues of algae was estimated. Methanolic extracts mes of seven brown seaweeds occurring in the indian coastal waters were screened for their cytotoxic and antioxidant properties following various assays. The larvicidal activity of brown algae padina minor. Padina tetrastromatica is one of the most important marine brown seaweed.
Antidiabetic, antihyperlipedimic and antioxidant activity of marine. In the typical synthesis of silver nanoparticles, 10 ml of the aqueous extract of padina tetrastromatica was added to 90 ml of 1 mm aqueous agno3 solution in 250 ml conical flask and kept at room temperature for 72 h at 120 rpm. Antibacterial and catalytic efficacy of biosynthesized. Sulfated polysaccharides from padina tetrastromatica arrest cell cycle, prevent metastasis and downregulate angiogenic mediators in hela cells assessment of oxidative stress indices in a marine macro brown alga padina tetrastromatica hauck from comparable polluted coastal regions of the arabian sea, west coast of india. Immune stimulatory and antihiv1 potential of extracts. High frap values for brown seaweeds sargassum polystum and padina sp. Novel antimrsa compounds from padina tetrastromatica. The larvicidal activity of brown algae padina minor yamada 1925 and dicyota linearis greville 1830 against the dengue vector, aedes aegypti linn 1762 diptera. During the last decade, fourier transform infrared ftir. Assessment of oxidative stress indices in a marine macro. Effect of ulva lactuca linn and padina tetrastromatica hauch concentrate on growth and yield of lablab purpureus linn. Dpph radical scavenging activity of padina tetrastromatica the dpph radical scavenging activity the dpph radical scavenging activity assay assessed the ability of the extract to donate hydrogen or to scavenge free radicals. Sulfated polysaccharides have shown promising effects on wound healing processes along with many other biological activities.
Burn injuries are the most common injuries and a major health problem affecting communities worldwide. The study revealed that slf of padina pavonia was found to be highly effective in enhancing only the growth and leaf area. The aim of this study is to adopt the approach of metabolic fingerprinting through the use of fourier transform infrared ftir technique to understand changes in the chemical structure in padina t. They were analysed by a hewlett packard 5890 gas chromatograph hewlett packard, palo alto, ca. The potential for using cyanobacteria bluegreen algae. Padina tetrastromatica sample collection and processing. Author links open overlay panel geena mariya jose g. The larvicidal activity of brown algae padina minor yamada. Uptake capacity of brown algal species padina tetrastromatica for heavy metals was evaluated in. The chemical composition of the brown alga padina pavonial. The effect of seaweed liquid fertilizer slf of ulva lactuca and padina tetrastromatica on biochemical constituents, antioxidants, growth and yield parameters such as number of leaves, weight of fruits in momordica charantia was examined. Ir spectroscopic study confirmed that the padina tetrastromatica extract has the ability to perform dual functions of reduction and stabilization of silver nanoparticles. A rapid and efficient dna extraction method suitable for.
The synthesis, characterization and application of biologically synthesized nanomaterials are an important aspect in nanotechnology. The complete reduction of silver ions was observed after 72 h of reaction at 300 oc under shaking. Hif1a expression in hela cells was also performed in the study. Heavy metals concentrations in brown seaweed padina pavonia l. Sulfated polysaccharides from padina tetrastromatica. Synthesis of silver nanoparticles silver nitrate agno3 was of analytical grade ar and purchased from e. The free radical scavenging acti biochemical composition in the present investigation total protein content varied from 7.
Cytotoxic effect of silver nanoparticles synthesized from. The bioactivities of edible brown algae padina tetrastromatica and green algae ulva fasciata are mainly attributed to the presence of sulfated polysaccharides spss. Pdf application of padina tetrastromatica for removal of. Jun 20, 2019 manfaat padina sp pdf makroalga cokelat laut padina tetrastromatica. The cellulose contents of indian seaweeds springerlink. In addition, acetone extracts of padina tetrastromatica and laurencia cruciata had the greatest inhibitory influence on p. Figure 1 from green synthesis of silver nanoparticles from. Ecofriendly synthesis and characterization of silver. The toxicological evaluation of a particular dose of a compound is necessary before practicing its pharmacological activities. Freeradicalscavenging activity and antioxidant effect of. However, investigations into the mechanisms of action pertaining.
The tandem mass spectrometric data revealed a series of phlorotannins with degree of polymerisation ranging from 2 to 18. Kulik soybean and alfalfa research laboratory, agricultural research service, united states department of agriculture, beltsville, md 20705, usa fax. This study illustrates that biosynthesis of silver nanoparticles agnps using fucoidan extracted from seaweed padina tetrastromatica. Pdf phytochemical screening of padina tetrastromatica hauck. Heavy metals concentrations in brown seaweed padina pavonia. Studies have shown that these seaweeds exhibit bioactivities which include antimicrobial, antiviral, antihypertensive and anticoagulant activities. Sep 29, 2015 three species of seaweeds padina tetrastromatica, caulerpa racemosa and turbinaria ornata are widely consumed by asians as nutraceutical food due to their antioxidant properties. Staphylococcus aureus is a grampositive pathogen responsible for causing several infectious diseases and has been shown to be one of the leading causes of. The compound was extracted and purified by using column. Jun, 20 read effect of ascophyllan from brown algae padina tetrastromatica on inflammation and oxidative stress in carrageenaninduced rats, inflammation on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Padina tetrastromatica hauck was collected along with the substratum without disturbing the holdfast, from anjuna 15 51n. Files are available under licenses specified on their description page. Figure 1 from green synthesis of silver nanoparticles. Oct 15, 2008 the aim of this study is to adopt the approach of metabolic fingerprinting through the use of fourier transform infrared ftir technique to understand changes in the chemical structure in padina tetrastromatica hauck. Biological synthesis of nanomaterials is a growing innovative approach and it was broadly utilised in the field of nanotechnology and nanomedicine. Reactive oxygen species ros include free radical for example. Application slf of padina tetrastromatica also enhancing number of leaves and above ground biomass. Health, general brown algae health aspects testing dengue prevention dengue fever public health vector control research yellow fever mosquito. The characteristic neutral loss of tandem mass spectra. Pdf application of padina tetrastromatica for removal. The potential for using cyanobacteria bluegreen algae and.
Cytotoxic effect of silver nanoparticles synthesized from padina tetrastromatica on breast cancer cell line b clara gnana selvi1, j madhavan2 and amutha santhanam3 1department of biotechnology, thiruvalluvar university, vellore632 115, india 2solar energy laboratory, department of chemistry, thiruvalluvar university, vellore632 115, india 3national centre for nanoscience and nanotechnology. Cytotoxic effect of silver nanoparticles synthesized from padina tetrastromatica on breast cancer cell line b clara gnana selvi1, j madhavan2 and amutha santhanam3 1department of biotechnology, thiruvalluvar university, vellore632 115, india. Ftir spectrum of padina tetrastromatica by various organic solvents acetone, ethanol. Jun 04, 2019 manfaat padina sp pdf makroalga cokelat laut padina tetrastromatica.
Biochemical composition, alginic acid yield and antioxidant. The presence of bands at 1627, 33 and 1032 cm1 indicates that agnps are possibly bound to proteins through carboxylate group. Padina tetrastromatica, antioxidant, antibacterial, gcms, phytochemical. Health, general brown algae health aspects testing dengue prevention dengue fever public health vector control research yellow fever mosquito control. Macro algae are nowadays used as a biosorbent material for removing heavy metals from wastewater. Carbohydrates of padina tetrastromatica pdf free download. All structured data from the file and property namespaces is available under the creative commons cc0 license. Antioxidant and cytotoxic activities of three species of tropical. Twelve sterols were identified in the sterol fraction, the main ones being cholesterol and fucosterol. Antibacterial and catalytic efficacy of biosynthesized silver. In context to hiv replication, the rt converts the singlestranded viral rna genome into proviral dna, which is the essential step prior to its integration into the host genomic dna. The in vitro antioxidant, antiproliferative, genotoxic and cytoprotective effects of the methanolic extract of the seaweed padina tetrastromatica were assayed using the 1,1diphenyl2picrylhydrazyl dpph radical, superoxide, nitric oxide and hydroxyl radical scavenging assays, 34,5dimethylthiazol2yl2,5diphenyltetrazolium bromide mtt and comet assays. Padina tetrastromatica, antioxidant, antibacterial, gcms, phytochemical correspondence to author. Thereafter, the cytotoxic effect of agnps against mcf7 breast cancer cell line has also been evaluated.
Effect of ascophyllan from brown algae padina tetrastromatica. Use of fourier transform infrared ftir spectroscopy to. The present study on padina tetrastromatica hauck fashioned a novel phytochemical marker in. Also, the phytochemical composition of the algae is known to possess a wide variety of bioactive compounds 11, 12. Antimrsa activity of padina tetrastromatica, padina. However, among the other brown algae, padina tetrastromatica got very low attention in terms of biomedical research.
Sulfated polysaccharides purified from two species of. Sulfated polysaccharides purified from two species of padina. Finally, the potential of lipid extracted residual biomass for bioethanol production through anaerobic fermentation is explored. Production of liquid biofuels biodiesel and bioethanol from. Pdf effect of ulva lactuca and padina tetrastromatica. In this study, phlorotannin from padina tetrastromatica brown macroalga was collected from mandapam, rameswaram, tamil nadu and identified by microscopic observation.
The present article reports a simple environmentally benign method for the synthesis of silver nanoparticles using aqueous extract of marine brown seaweed padina tetrastromatica, which act as both the reducing and stabilising agent. Antibacterial and catalytic efficacy of biosynthesized silver nanoparticles using marine seaweed padina tetrasromatica. The dpph radicle is a stable free radicle and when it reacts with an antioxidant compound. Pdf nowadays marine algae are having a numerous commercial applications in various fields. Total free amino acid content of freshly collected frozen tissues of algae was estimated by ninhydrin method moore and stein, 1948. Assistant professor, guru nanak college, chennai, india. Keywords padina tetrastromatica, sulfated polysaccharides. The high protein content was recorded in hormophysa triquetra 15. Figure 4 from green synthesis of silver nanoparticles. Attenuation of hydrogenperoxideinduced oxidative damages in. Padina tetrastromatica was a misapplied name for p. Pdf the present report deals with the study of phytochemicals present in padina tetrastromatica hauck.
Chemical composition of the brown alga padina pavonia l. Sulfated polysaccharides from padina tetrastromatica arrest cell cycle, prevent metastasis and downregulate angiogenic mediators in hela cells. The methanolic extracts of seaweeds in the order of dictyopteris australis spatoglossum variabile stoechospermum marginatum spatoglossum aspermum showed significant cytotoxic activity. The sulfated polysaccharides extracted from two algae species habitats in persian gulf were studied in vivo for their effects. Apr 12, 2010 read polysaccharides from padina tetrastromatica. Phlorotannins are the phenolic compounds which can inhibit the oxidative stress via scavenging the free radicals. Seaweeds, being abundant sources of active components have attained much interest in recent times. Structural features, chemical modification and antiviral activity, carbohydrate polymers on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Mar 18, 2019 manfaat padina sp pdf makroalga cokelat laut padina tetrastromatica. Free radicalscavenging activity and antioxidant effect of ascophyllan from marine brown algae padina tetrastromatica. The applicability of the conductometric method for controlling the ion exchange process is shown not only when the free cations are isolated from aqueous solutions but also bound to. After the original description of the species, the only reports of p.
Manfaat padina sp pdf makroalga cokelat laut padina tetrastromatica. Four polysaccharide fractions were purified from crude. Comparative photocatalytic degradation of organic dyes. Utilization of seaweeds to enhance growth and nutritive.
Hence, in the present study, an effort is made to biosynthesize agnps using the seaweed extract of padina tetrastromatica. Heavy metals concentrations in brown seaweed padina. Collection was made by snorkeling and transported to the laboratory in polyethene containers containing seawater. The algal samples were studied using fourier transform infrared ftir technique. Ic50 values in free radical scavenging assays for extracts of p. Marine algae, padina tetrastromatica, antimicrobial activity, brown.
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